Faculty Rank

Professor

Undergraduate Education

Philadelphia College of Pharmacy and Science, B.S., 1964

Graduate Education

University of Pennsylvania, The Wistar Institute, Ph.D., 1969

Postdoctoral

University of Pennsylvania, The Wistar Institute, 1970

University of Colorado School of Medicine, 1971

Research Interests

Laboratory research has focused on an understanding of the interaction of the SV40 virus large T antigen and small t antigen with cell cycle regulatory proteins and how these proteins are involved in cell transformation and cell cycle dysregulation.  These studies will form a basis for our understanding the regulation of the G2 and S phase of the cell cycle and how the SV40 antigens (T and t) modulate this regulation leading to the stimulation of cell DNA synthesis, a block to mitosis and second S phase (tetraploidy-polyploidy).

Selected Publications

1.  Friedrich TD, Bender E, Darzynkiewicz Z, Lehman JM. Distinct Patterns of MCM Protein Binding in Nuclei of S Phase and Rereplicating SV40-infected Monkey Kidney Cells. Cytometry. 2005;67A:10-18.

2.  Dee FR, Lehman JM, Consoer D, Leaven T, Cohen MB. Implementation of Virtual Microscope Slides in the Annual Pathobiology of Cancer Workshop Laboratory: Human Pathology. 2003;34:430-436.

3.  Okubo E, Lehman JM and Friedrich TD. Negative regulation of mitotic promoting factor by the checkpoint kinase Chk1 in Simian Virus 40 lytici infection.  J of Virology. 2003;77:1257-1267.

4.  Zhang Y, Lehman JM, Petti LM. Apoptosis of mortal human fibroblasts transformed by the bovine papillomavirus E5 oncoprotein. Molecular Cancer Research. 2002;1:122-136.

5.  Lehman JM, Laffin J and Friedrich TD. Simian Virus 40 induces multiple S phases with the majority of viral DNA replication in the G2 and second S phase in CV-1 cells. Exp Cell Res. 2000;258:215-222.

6.  Whalen B, Laffin J, Friedrich TD and Lehman JM. SV40 small t antigen enhances rereplication and alters expression of G1 regulatory proteins in permissive CV1 cells. Exp Cell Res. 1999;251:121-127.

7.  Albano-Perry MB and Lehman JM. Activities of SV40 T antigen necessary for induction of tetraploidy in permissive CV1 cells. Cytometry. 1998;31:251-259.

8.  Lehman JM, Albano MB and Laffin J. Induction of tetraploid DNA content by mutant Simian virus 40 T antigen which has reduced complex formation with p53. Cytometry. 1996;23:126-130.

9.  Kulju K and Lehman JM. Increased p53 protein associated with aging in human diploid fibroblasts. Exp Cell Res. 1995;217:336-345.

10. Friedrich TD, Laffin J and Lehman JM. Induction of tetraploid DNA content by Simian virus 40 is dependent on a T antigen function in G2 phase of the cell cycle.  J Virology. 1994;68:4028-4030.

11. Scarano FJ, Laffin J, Lehman JM and Friedrich TD. Simian virus 40 prevents activation of M-phase promoting factor during lytic infection. J Virol. 1994;68:2355-2361.