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Faculty Rank
Professor
Undergraduate Education
Philadelphia
College of Pharmacy and Science, B.S., 1964
Graduate Education
University
of Pennsylvania, The Wistar Institute, Ph.D., 1969
Postdoctoral
University
of Pennsylvania, The Wistar Institute, 1970
University of Colorado School of
Medicine, 1971
Research
Interests
Laboratory research has focused on an understanding of
the interaction of the SV40 virus large T antigen and
small t antigen with cell cycle regulatory proteins and
how these proteins are involved in cell transformation
and cell cycle dysregulation. These studies will
form a basis for our understanding the regulation of the
G2 and S phase of the cell cycle and how the SV40
antigens (T and t) modulate this regulation leading to
the stimulation of cell DNA synthesis, a block to
mitosis and second S phase (tetraploidy-polyploidy).
Selected
Publications
1. Friedrich TD, Bender E, Darzynkiewicz Z, Lehman JM.
Distinct Patterns of MCM Protein Binding in Nuclei of S
Phase and Rereplicating SV40-infected Monkey Kidney
Cells. Cytometry. 2005;67A:10-18.
2.
Dee FR, Lehman JM, Consoer D, Leaven T, Cohen MB.
Implementation of Virtual Microscope Slides in the
Annual Pathobiology of Cancer Workshop Laboratory:
Human Pathology. 2003;34:430-436.
3. Okubo E, Lehman JM
and Friedrich TD. Negative regulation of mitotic
promoting factor by the checkpoint kinase Chk1 in Simian
Virus 40 lytici infection. J of Virology.
2003;77:1257-1267.
4. Zhang Y, Lehman JM,
Petti LM. Apoptosis of mortal human fibroblasts
transformed by the bovine papillomavirus E5 oncoprotein.
Molecular Cancer Research. 2002;1:122-136.
5. Lehman JM, Laffin J
and Friedrich TD. Simian Virus 40 induces multiple S
phases with the majority of viral DNA replication in the
G2 and second S phase in CV-1 cells. Exp Cell Res.
2000;258:215-222.
6. Whalen B, Laffin J,
Friedrich TD and Lehman JM. SV40 small t antigen
enhances rereplication and alters expression of G1
regulatory proteins in permissive CV1 cells. Exp Cell
Res. 1999;251:121-127.
7. Albano-Perry MB and
Lehman JM. Activities of SV40 T antigen necessary
for induction of tetraploidy in permissive CV1 cells.
Cytometry. 1998;31:251-259.
8. Lehman JM, Albano MB
and Laffin J. Induction of tetraploid DNA content by
mutant Simian virus 40 T antigen which has reduced
complex formation with p53. Cytometry.
1996;23:126-130.
9. Kulju K and Lehman JM.
Increased p53 protein associated with aging in human
diploid fibroblasts. Exp Cell Res.
1995;217:336-345.
10. Friedrich TD, Laffin J and
Lehman JM. Induction of tetraploid DNA content by
Simian virus 40 is dependent on a T antigen function in
G2 phase of the cell cycle. J Virology.
1994;68:4028-4030.
11. Scarano FJ, Laffin J, Lehman
JM and Friedrich TD. Simian virus 40 prevents
activation of M-phase promoting factor during lytic
infection. J Virol. 1994;68:2355-2361.
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